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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Mol Carcinog. 2012 Dec 19;53(5):403–412. doi: 10.1002/mc.21988

Figure 2.

Figure 2

Shikonin (SKN) inhibited tumor promoter TPA-induced skin cell transformation. The final concentration of TPA was 5 nM. Two concentrations (0.2 and 0.4 μM) of shikonin were used. (A) Soft agar colony formation of JB6 P+ cells (light micrograph: 10× magnification). (B) Quantification of formed colonies. Cells grown in 0.33% soft agar containing 5 nM TPA and/or 0.2 or 0.4 shikonin or control (DMSO 1000× diluted). *P < 0.05 compared with the control/DMSO group; #P < 0.05 compared with the TPA group (n = 3 in each group).