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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Mol Carcinog. 2012 Dec 19;53(5):403–412. doi: 10.1002/mc.21988

Figure 6.

Figure 6

Shikonin (SKN) inhibited cell growth through causing G2–M accumulation and suppressing Fra-1 protein expression in JB6 P+ cells. (A)–(C) Cell cycle analysis. Cells were starved in serum-free EMEM medium for 24 h and then treated with TPA (100 nM) or shikonin (1 μM) for 24 h. Cell cycle analysis was performed by flow cytometry. (D) Western blot to detect Fra-1 expression. Cells were treated with TPA (100 nM) or shikonin (1 μM) for 24 h. The level of Fra-1 was normalized to that of β-actin. *P < 0.05 compared with the control (DMSO) treatment; #P < 0.05 compared with the TPA treatment.