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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Bone Miner Res. 2015 Sep 23;31(2):430–442. doi: 10.1002/jbmr.2690

Figure 5. IDG-CM6 cementocytes express E11/gp38.

Figure 5

Immunostaining for E11 in 3 month old mouse tissues shows localization in (A) new osteocytes in the tibia, with less expression in (B) new osteocytes of alveolar bone (AB) bordering the periodontal ligament (PDL), and (C) new cementocytes in cellular cementum (CC). Positively stained cells in panels A-C are indicated by black arrows. (D) Quantitative PCR from tissues confirms expression of E11/gp38 mRNA in long bone (LB), alveolar bone (AB), cellular cementum (CC), and molar and incisor pulp (MP and IP, respectively) (n=4 for each tissue, Ct values 27 – 33). No significant intergroup differences were detected by one-way ANOVA, as indicated by same capital letters above tissue types. (E-H) Both IDG-CM6 and IDG-SW3 express E11/gp38 mRNA and protein over the course of 42 days in mineralizing media, in vitro. E11/gp38 mRNA expression from primary dental pulp (DP) is included in panels E and F as a reference for comparison to cells, in vitro. (Ct values for IDG-CM6: 25-27, IDG-SW3: 24-29) In D-F, data are presented as mean ± SD. One-way ANOVA and Tukey post-hoc test were used to indicate inter- or intragroup statistical significance. Different lowercase letters above bars indicate significant (p < 0.05) intragroup differences over time.