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. 2015 Nov 4;1(8):431–438. doi: 10.1021/acscentsci.5b00259

Figure 4.

Figure 4

A nanotag based on antibody tethered to the DBBP (a) A DBBP with a single specific sequence B can be hybridized to Cy5-Acomp and antibody bearing fully complementary B′comp strand and loaded with YOYO-1. The single sequence B per brush is to ensure one antibody per brush. (b) The c-myc protein detection system using primary and secondary antibody interactions where the tag (X) is on the secondary antibody. (c) Flow cytometry data shows high fluorescent brightness of the brush nanotag compared to other commercially available antibody tags. (d) Confocal microscopic images show the exceptional brightness of the nanotag antibodies compared to Alexa 647 and QD655 tagged antibodies. (e) Detection of maltose binding protein (MBP) using the primary and secondary antibody system (left) and the visual confirmation of the specificity and brightness of DBBP nanotags via dot blots. The nanotag antibodies allow visualization with at least an order of magnitude greater sensitivity. See Supporting Information for additional imaging and quantitation.