Table I.
Information for the studied markers in the VDR, PTH, and INSR genes
|
Gene
(SNP) |
SNP reference number |
Location
(Base change) |
Forward primer
Reverse primer |
PCR
condition |
PCR
fragment size (bp) |
Restriction
enzyme, Incubation temperature |
RFLP
fragments size (bp) |
|---|---|---|---|---|---|---|---|
| VDR (FokI) |
rs10735810 | Exon 2 (C/T) |
5'-AGCTGGCCCTGGCACTGACTCTGCTCT-3' 5'-ATGGAAACACCTTGCTTCTTCTCCCTC-3' |
35 cycles 93C 45s, 66C 30s, 72C 45s |
265 | FoKI, 55C | 169, 96 |
| VDR (BsmI) |
rs1544410 | Intron 8 (A/G) |
5’-GGCAACCTGAAGGGAGACGTA-3’ 5’-CTCTTTGGACCTCATCACCGAC-3’ |
35 cycles 93C 45s, 66C 30s, 72C 45s | 461 | BsmI, 37C | 258, 203 |
| VDR (ApaI) |
rs7975232 | Intron 8 (A/C) |
5’-CAGAGCATGGACAGGGAGCAAG-3’ 5’-GCAACTCCTCATGGCTGAGGTCTCA-3’ |
35 cycles 93C 45s, 66C 30s, 72C 45s | 740 | ApaI, 37C | 530, 210 |
| VDR (Tru9I) |
rs757343 | Intron 8 (A/G) |
5’-AATACTCAGGCTCTGCTCTT-3’ 5’-CATCTCCATTCCTTGAGCCT-3’ |
35 cycles 93C 45s, 56C 30s, 72C 45s |
331 | Tru9I, 65C | 178, 153 |
| VDR (TaqI) |
rs731236 | Exon 9 (C/T) |
5’-CAGAGCATGGACAGGGAGCAAG-3’ 5’- GCAACTCCTCATGGCTGAGGTCTCA-3’ |
35 cycles 93C 45s, 66C 30s, 72C 45s | 740 | TaqI, 65C | 495,290, 245, 205 |
| PTH (DraII) |
rs6256 | Exon 3 (A/C) |
5’-CATTCTGTGTACTATAGTTTG-3’ 5’-GAGCTTTGAATTAGCAGCATG-3’ |
35 cycles 93C 45s, 56C 30s, 72C 45s |
600 | DraII, 37C | 420, 180 |
| INSR (NsiI) |
rs2059806 | Exon 8 (A/G) |
5’-CGGTCTTGTAAGGGTAACTG-3’ 5’-GAATTCACATTCCCAAGACA-3’ |
35 cycles 93C 45s, 56C 30s, 72C 45s | 324 | NsiI, 37C | 239, 85 |
| INSR (PmlI) |
rs1799817 | Exon 17 (C/T) |
5’-CCAAGGATGCTGTGTAGATAAG-3’ 5’-TCAGGAAAGCCAGCCCATGTC-3’ |
35 cycles 93C 45s, 56C 30s, 72C 45s | 317 | PmlI, 37C | 274, 43 |
VDR: Vitamin D receptor; PTH: Parathyroid hormone; INSR: Insulin receptor.