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. 2016 Apr 5;11:1413–1425. doi: 10.2147/IJN.S95850

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© 2016 Han et al. This work is published and licensed by Dove Medical Press Limited

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Isolation and characterization of BCSCs.

Notes: (A) Morphology of MCF-7 cells and BCSCs, observed using a bright-field microscope (scale bar =200 μm). (B) Flow cytometric analysis of the cells for CD24⁻ and CD44⁺ population. (C) Percentages of secondary sphere formation from the single cells from MCF-7 cells or BCSCs. (D) Representative photographs of secondary spheres (scale bar =100 μm) (the blue arrow indicates a single cell, which didn’t form a sphere). Data shown represent the mean ± SD of three experiments (^**P<0.01).

Abbreviations: BCSCs, breast cancer stem cells; SD, standard deviation; FITC, fluorescein isothiocyanate; PE, phycoerythrin.