Toxicity of Ugp1 overexpression is rescued by high copy suppressors from an S. cerevisiae pRS426-based library. The PSK1psk2 (JRY277) strain overexpressing UGP1 from a trp plasmid (pJR3020b) displays a galts growth defect that may be rescued by high copy suppressors (HCS) from a pRS426-based library. All samples have UGP1 overexpreessed and the suppressors are shown in comparison to the empty pRS426 vector control. Samples are, from top to bottom, pRS426 control, PSK1, PGM2, ORM1, MNN11, TIM44 and ERP3. Samples are shown in dilution series from left to right and were prepared as described in Figure 3 and plated on SGal-trp-ura at 30°C for 3 days.