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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Sci Transl Med. 2015 Jul 29;7(298):298ra117. doi: 10.1126/scitranslmed.aaa7619

Figure 3. Low dose dietary resveratrol activates AMPK and causes senescence in intestinal mucosa of mice on HFD.

Figure 3

(a–c) Expression and phosphorylation of AMPK and its downstream target ACC, together with levels of autophagy and senescence markers in tissue of female ApcMin mice maintained on SD or HFD, with or without resveratrol. The positive control sample is Apc10.1 cells exposed to 1 μM resveratrol. Mice were culled at 17 or 14 weeks of age for the standard and high fat groups, respectively. (c) Data represent the mean ± SEM of 6 mice per group. (d–e) Kinetics of AMPK activation and downstream effects in intestinal tissue of C57BL/6J wild-type male mice maintained on HFD which received a single gavage dose of resveratrol (2.1 μg per mouse; R) or vehicle control (C). Mice were culled post-dosing at the indicated time. (d) Representative immunoblots are shown for 3 mice per group. (e) Data represent the mean ± SEM of 4–6 mice per group.