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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Exp Physiol. 2016 Feb 25;101(4):494–508. doi: 10.1113/EP085405

Figure 7. Female cardiac real-time PCR expression, protein and protein–protein interaction analysis.

Figure 7

A, expression of myosin heavy chain (MHC) isoforms α and β, PI3K, GATA4, GATA6 and BMP10 (known cardiac specific hypertrophy markers); bar graph shows means ± SEM (n = 3), and *P < 0.05. Genes were normalized with a housekeeping gene (HPRT). B, Western blot images of MHCα and MHCβ from WT and KO left ventricular homogenate. C, bar graph shows means ± SEM (n = 3), and *P < 0.05; bands were normalized with Ponceau S-stained full lanes. D, Western blot image of KO left ventricle (Lv) and Cos-7 cells transfected with Kvβ1.1–DDK plasmid (β1.1DDK; lane 1) and Cos-7 cells transfected with β1.1DDK alone (lane 2). Lane 1 was incubated overnight with DDK-coated agarose beads. Myosin heavy chain α (MHCα) primary antibody (1:200 dilution) was incubated overnight with blot, and a 225 kDa band was noted in lane 1, with a limited band seen in lane 2. E, PCR expression of MHCα, GATA4 and GATA6 72 h after Kvβ1.1 small interfering RNA treatment in H9C2 cells (rat cardiomyoblasts); bar graph shows means ± SEM (n = 3), and *P < 0.05. †P < 0.07.