Table 1.
Assessment of input factors. Qualitative assessment ranged from +++ (very good) to + (poor). The input factors (A)–(C) were ultimately selected (bold text).
| Input factor | Assumed effectiveness | Independent variability | Variability at representative factor levels | Quantitative variability |
|---|---|---|---|---|
| (A) ΔG of the MP-target heterodimer | +++ | + | +++ | +++ |
| (B) ΔG of the mediator-UR heterodimer | +++ | + | +++ | +++ |
| (C) Distance between primer and MP cleavage site | +++ | + | +++ | +++ |
| (D) Mediator length | +++ | + | ++ | +++ |
| (E) 3′-terminal base at mediator | + | + | + | + |
| (F) Mediator binding position at UR | ++ | + | + | +++ |
| (G) Sequence around MP cleavage site | ++ | + | + | + |