Table 3.

Results obtained by multiplex PCR and conventional methods (TLC and PCR)

Isolate code	Aflatoxin biosynthesis gene					Aflatoxin production by TLC method
	aflR	omt-A	ver-1	nor-1		Aflatoxin production
SAF1	+	+	+	+		+
SAP1	-	-	+	+		-
SAP2	+	+	+	+		+
CAF1	+	+	+	+		+
CAF2	+	+	-	+		-
CAF3	-	-	-	+		-
CAF4	-	+	-	+		-
CAF5	+	+	+	+		+
CAF6	-	-	-	+		-
CAF7	+	+	+	+		+
CAF8	-	-	+	+		-
CAP1	+	+	+	+		+
CAP2	+	+	+	+		+
CAP3	+	+	-	+		-
TAF1	-	-	-	+		-
TAF2	-	-	+	+		-
TAF3	-	-	+	+		-
TAF4	+	+	+	+		+
TAF5	-	-	+	+		-
TAP1	+	+	+	+		+
TAP2	-	-	+	+		-
HAF1	-	-	+	+		-
HAF2	-	-	-	+		-
HAF3	-	-	+	+		-
HAF4	-	+	-	-		-
HAF5	+	+	-	+		-
HAP1	-	-	+	+		-
HAP2	+	+	+	+		+
A. niger ATCC 9142	-	-	-	-		-
F. oxysporum PTCC 5115	-	-	-	-		-
A. parasiticus ATCC15517	+	+	+	+		+
P. purporogenome PTCC 5212	-	-	-	-		-

S: Silage, C: Concentrate, T: TMR, H: Hay, AF: A. flavus, and AP: A. parasiticus