Table 1.
Yield and purity of extracted DNA (mean ± SD). Six different treatment groups, (SF-snap frozen, 60 min SF and 300 min SF-left in room temperature for the indicated time to allow for post-sampling changes to appear, HS-heat stabilized, HS + 60 min and HS + 300 min-heat stabilized with incubation at room temperature after heat stabilization for the indicated time). A260/A280-assesment of protein contamination, A260/A230-assesment of contaminants such as guanidine thiocynate and carbohydrates. **p < 0.01 compared with snap frozen samples (SF), ##p < 0.01, compared to corresponding time in room temperature for snap frozen samples (ANOVA followed by Tukey HSD test test; n = 4).
| Group | μg DNA/mg sample | 260/280 | 260/230 |
|---|---|---|---|
| SF | 892 ± 132 | 1.91 ± 0.01 | 2.30 ± 0.06 |
| 60 min SF | 827 ± 247 | 1.90 ± 0.01 | 2.26 ± 0.10 |
| 300 min SF | 733 ± 324 | 1.91 ± 0.00 | 2.58 ± 0.53 |
| HS | 1367 ± 320 | 1.90 ± 0.01 | 2.23 ± 0.03 |
| HS + 60 min | 2502 ± 1057**,## | 1.90 ± 0.03 | 2.11 ± 0.16 |
| HS + 300 min | 2510 ± 871**,## | 1.90 ± 0.00 | 2.22 ± 0.08 |