Table 1.
Expression changes of selected genes in the 1021Δhfq mutanta
| Relative expression ratio 1021 Δ hfq mutant vs. Rm1021 |
||
|---|---|---|
| Gene | SymbiosisChipb | Real-time PCRc |
| SMc02118 (aapJ) | 2.2 | 4.1 ± 0.4 |
| SMc02119 (aapQ) | 4.1 | 11.0 ± 0.6 |
| SMc02120 (aapM) | 4.8 | 8.4 ± 0.4 |
| SMc01946 (livK) | 5.5 | 14.0 ± 0.3 |
| SMc01948 (livF) | 11.0 | 11.3 ± 0.3 |
| SMc01949 (livG) | 10 | 10.9 ± 0.4 |
| SMc01950 (livM) | 13.9 | 18.6 ± 0.4 |
| SMc03201 (bkdAa) | 5.4↓ | (15.0 ± 3.0)4↓ |
| SMc03202 (bkdAb) | 5.4↓ | (6.0 ± 1.0)4↓ |
| SMc03203 (bkdB) | 5.7↓ | (9.2 ± 0.6)4↓ |
| SMc00168 (sinI) | 1.8 | (2.6 ± 0.3) |
| SMc02584 (actR) | 3.1 | 4.8 ± 0.6 |
| SMc02585 (actS) | 3.5 | 4.7 ± 0.2 |
| SMc02484 (supA) | 6.34↓ | (13 ± 1.0)4↓ |
| Smc02485 (supB) | 4.34↓ | (16 ± 2.0)4↓ |
Downward arrows indicate decreased fold change in the 1021Δhfq mutant. Cultures for real-time polymerase chain reaction (PCR) were grown under the same conditions as, but independently of, those used for Symbiosis-Chip analysis.
Fold change is based on signal log ratio value from the SymbiosisChip data.
Fold change is calculated from cycle threshold (Ct) values obtained from real-time PCR experiments. The SMc00129 RNA was used an internal control. The Ct values of the internal control for the Rm1021 and 1021Δhfq were 28.64 and 28.67 for the aap and liv experiment, 31.43 and 31.77 for the sup experiment, 25.59 and 25.52 for the bkd qPCR experiment, and 25.81 and 26.46 for the sinI/act experiment, respectively. The standard errors of the means from qPCR experiments are shown (n = 3).