Figure 1.

Workflow of the primary stage. This primary stage consists of four parts that include preparation of tissue materials, creation of sequencing template, perform imaging, and sequencing. Their unique combination determines how the genetic sequence information is generated from tissues of different organisms. Although the focus of this stage of workflow is on the latter three parts, the importance of selection and preparation of tissue materials for NGS analysis cannot be neglected. The biased preparation of tissue materials could lead to fatal result of NGS workflow.