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. 2016 Apr 11;12(4):e1005556. doi: 10.1371/journal.ppat.1005556

Fig 11. Knock-down of NCoR/SMRT components by siRNA increases transcription of HPV31 genomes.

Fig 11

SCC13 cells were transfected with a control (siCon) or specific siRNAs (90pmol) and the next day with 1.3μg of re-ligated HPV31 wt (wt) or HPV31 E8 KWK mt (E8 KWK mt) genomes. RNA was isolated 48h later and analyzed by qPCR for the expression of spliced E1^E4, HDAC3, NCoR, SMRT, TBLR1 and PGK1 as a reference using specific primer pairs. Data are derived from four independent experiments and the values are presented relative to HPV31 wt/siControl. Error bars represent the SEM and statistical significance was tested by 1way ANOVA and Bonferroni´s multiple comparison test as a post test (*p<0.05).