Fig 12. DN-NCOR activates viral transcription.

(A) RTS3b cells were transfected with the pSG-DN-NCoR plasmid and stained for DN-NCoR expression using an anti-Flag antibody by indirect immunofluorescence. DAPI was used to identify cell nuclei. (B) RTS3b cells were transfected with the reporter plasmid pC18-Sp1-luc (100ng) and expression vectors for HPV31 E8^E2C, E8^E2C KWK mt (10 ng) and DN-NCoR as indicated and pCMV-Gluc as a transfection control: Data are derived from three independent transfection experiments carried out in duplicate and are presented relative to pC18-Sp1-luc/empty vector. Error bars indicate the SEM. (C) SCC13 cells were transfected with 1.3μg each of HPV16 wt, HPV16 E8 KWK mt, HPV31 wt or HPV31 E8 KWK mt genomes and 0.5μg of the empty vector (vec) or pSG DN-NCoR plasmid. RNA was analyzed by qPCR using E1^E4 and PGK1 primers. Data are derived from five independent experiments and are presented relative to HPV wt/empty vector (vec). Error bars represent the SEM and statistical significance was tested by 1way ANOVA and Bonferroni´s multiple comparison test as a post test (*p<0.05; **p<0.01).