Table 1. Purification of cupincin from rice bran*.
Steps | Total activity (units) | Total protein (mg) | Specific activity(units/mg) | Purification (Fold) | Recovery (%) |
---|---|---|---|---|---|
Crude | 713.4 | 1771.2 | 0.40 | 1 | 100 |
80% (NH4)2SO4 precipitation | 411.25 | 384.88 | 1.06 | 2.65 | 21.72 |
Sephadex G-150 size exclusion chromatography | 155.06 | 86.77 | 1.78 | 4.43 | 4.9 |
* These are typical purification results from 50 g of rice bran. These values are reproduced in three separate purifications. Denatured haemoglobin was used as the substrate for activity measurements.