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. 2016 Apr 11;11(4):e0152819. doi: 10.1371/journal.pone.0152819

Table 1. Purification of cupincin from rice bran*.

Steps Total activity (units) Total protein (mg) Specific activity(units/mg) Purification (Fold) Recovery (%)
Crude 713.4 1771.2 0.40 1 100
80% (NH4)2SO4 precipitation 411.25 384.88 1.06 2.65 21.72
Sephadex G-150 size exclusion chromatography 155.06 86.77 1.78 4.43 4.9

* These are typical purification results from 50 g of rice bran. These values are reproduced in three separate purifications. Denatured haemoglobin was used as the substrate for activity measurements.