Fig 1. Effects of E2 on the differentiation of DC from BM.

BM cell isolated from OVX mice were cultured in sex hormone-deficient medium with murine GM-CSF alone, or in the presence of varying concentrations of E2 (A, B, C, D). Initial gating for mononuclear cells is shown in (A). Data in (B, C, D) are from cells collected at end of culture, and stained with Abs for CD11b and CD11c. Representative dot plot data are shown in (B). Data for percentages of CD11b⁺CD11c⁺ DCs from cultures with different concentrations of E2 and treated with (dashed) or without (solid) LPS are shown in (C). Absolute numbers of CD11b⁺ CD11c⁺ DCs cultured with different concentrations of E2 are shown in (D). Data shown is Mean ± SD of 6 independent experiments. Statistical significance is indicated by * p<0.05; **p<0.01; *** p<0.001 compared with untreated control.