Fig 2. Effects of P4 on the differentiation of APCs from BM.

BM cell isolated from OVX mice were cultured in sex hormone-deficient medium with murine GM-CSF alone, or in the presence of varying concentrations of P4. Data in (A, B, C) are from cells collected at end of culture, and stained with Abs for CD11b and CD11c. Initial gating for monocytes as shown in Fig 1A. Representative dot plot data are shown in (A). Percentages of CD11b⁺CD11c⁺ APCs from cultures grown in presence of different concentrations of P4, treated with (dashed) or without (solid) LPS are shown in (B). Absolute numbers of CD11b⁺ CD11c⁺ APCs cultured with P4 are shown in (C). Data show Mean ± SD of 6 independent experiments. Statistical significance is indicated by * p<0.05; **p<0.01; *** p<0.001 compared with untreated control.