Fig 4. High doses of P4 reversed effects of E2 in presence or absence of LPS treatment.

BM cells isolated from OVX mice were cultured with GM-CSF in the presence of E2 at a low (10^-11M) (A) or high (10^-9M) (B) concentration with varying doses of P4 (10^-9M to 10^-5M), with (dashed) or without (solid) LPS. At the end of culture, cells were harvested and stained with Abs to detect CD11b, CD11c, CD40 and MHCII. Data are percentages of CD11b⁺CD11c⁺ DCs (top panels); percentages of CD40⁺/CD11b⁺CD11c⁺DCs (middle panels); and percentages of MHCII⁺/CD11b⁺CD11c⁺ DCs (bottom panels). Data shown are Mean ± SD of 6 independent experiments. Statistical significance is indicated by *p<0.05; **p<0.01;*** p<0.001 compared with E2 only treated controls.