Fig 7. Expression pattern of mating-type, HO and IME4 genes.
Panel A reports positive amplified cDNAs generated with MAT and HO copy variant-specific primers from ATCC 42981 unstressed cells in stationary growth phase. Panel B depicts IME4-specific PCR products generated from CBS 732T and ATCC 42981 cDNA IME4 antisense (AS-IME4 lncRNA) and sense transcripts (S-IME4 mRNA), respectively. +/- RT indicates addition of reverse transcriptase to the cDNA synthesis reaction. For each RT-PCR reaction gDNA was used as positive control. Abbreviations: AS, anti-sense long non-coding RNA; S, sense mRNA.