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. 2016 Apr 11;12(4):e1005537. doi: 10.1371/journal.ppat.1005537

Fig 1. Targeted isolation of SIV CD4bs-specific B cells.

Fig 1

Schematic illustrating competitive probe binding strategy for targeted isolation of CD4bs-specific B cells. Cells are first stained with a CD4bs-occluded probe (i.e. CD4-Ig:SIVgp140-PE) to label all SIV-specific B cells (red) except those that are specific for the CD4bs (blue) (Step 1) as shown in FACS plot (top right). After extensive washing to remove unbound CD4-Ig:SIVgp140-PE from first stain (Step 1), cells are stained with a CD4bs-accessible probe (i.e. SIVgp140-APC in the absence of any competing CD4-Ig) (Step 3). All SIV-specific B cells, including those specific for the CD4bs, will bind to this probe with putative CD4bs-specific B cells (blue) being positive for SIVgp140-APC and negative for CD4-Ig:SIVgp140-PE as shown in FACS plot (bottom right).