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. 2016 Apr 11;12(4):e1005537. doi: 10.1371/journal.ppat.1005537

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Fig 2 — A) Gating strategy for isolating rhesus macaque memory B cells i.e. lymphocytes / singlets / Live / CD3-CD4^-CD8^-CD14^- / CD20⁺ / IgM^- / IgG⁺ and B) FACS data overlay of individually sorted CD4bs-specific B cells (multicolored) as a percentage of total memory B cells (gray). C) Competition ELISA of biotinylated CD4-Ig binding to plate-bound SIVmac239 gp140 FT in the presence of individual competing mAbs graphed as percent inhibition = (OD without competitor—OD with competitor)/ OD without competitor X100. D) ELISA of non-binding mAbs at 5 μg/ml to plate-bound SIVmac239 gp140 FT in the presence of varying concentrations of sCD4.