Skip to main content
. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: J Transl Sci. 2016 Mar 5;2(2):117–124. doi: 10.15761/JTS.1000127

Figure 4.

Figure 4

Inhibition of DNA/RNA syntheses and induction of γH2AX by cisplatin, mitomycin C, triapine or paclitaxel. Panels A, for short-term treatment, HL-60 cells (2 × 106 cells/ml) were treated with various agents for 2 h, and labeled with 3H-thymidine or 3H-uridine for 1 h. For long-term treatment, HL-60 cells (4 × 105 cells/ml) were treated with various agents for 18 h, condensed to 2 × 106 cells/ml and labeled with 3H-thymidine or 3H-uridine for 1 h. Panels B, HL-60 cells (4 × 105 cells/ml) were treated with various agents for 18 h. Histones extracted from intact cells were subjected to 15% PAGE and western analyses using an anti-γH2AX antibody. For a loading control, duplicate gels were used to stain core histones with GelCode Blue Safe Protein Stain (Thermo Scientific).