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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: J Mol Cell Cardiol. 2015 Jan 7;80:114–125. doi: 10.1016/j.yjmcc.2014.12.021

Figure 1. Perfusion protocols.

Figure 1

Hearts from 10-14 week old C57/Bl6 male mouse were studied. Protocol A was used to assess trigger phase signaling (PI3K, Akt and PKCε). Protocol B was used to evaluate ischemia/reperfusion (IR) injury. After equilibration, IR was triggered by 40 min of zero-flow ischemia followed by 30 min of reperfusion, unless otherwise stated. Ischemic preconditioning (IPC) was induced by 4 cycles of 5min zero-flow ischemia and 5 min reperfusion. Ouabain preconditioning (OPC) was induced by 4min of exposure to ouabain 10μM followed by 8min washout before ischemia. KH: Krebs Henseleit buffer.