Figure 4.

The C-terminal domain of FMRP is able to specifically interact with mRNA from the indicated subunits of the GABA_A receptor. In each electrophoretic mobility shift assay (EMSA), ³²P-labeled mRNA-fragments were incubated alone (lane 1), in the presence of BSA (lane 2) or in the presence of the C-terminal (CT) domain of FMRP (lane 3). Direct interaction of the labeled mRNA with FMRP causes a shifted band in the third line (lane A3, B3, C3, D3 and E3) since the protein:RNA complexes migrate more slowly on the polyacrylamide gel. Known targets of FMRP were used as positive controls, PSD-95 3'UTR-fragment 5 (D) ⁵ and fragment N19 of FMR1 (E) ¹¹. (F) PSD-95 3'UTR-fragment 1 ⁵ was used a negative control. Unbound RNA-fragments ([) and protein:RNA complexes (*) are indicated.