Diagram of the separation system of CCC instruments. The separation system was constructed by 5 parts (Sample injection of 5 ml in an ultra-sound both, Separation funnel, HPLC pump, Fraction collector, Recorder). The length of column tube was 12 m and the inside diameter was 1.6 mm, the total volume was 33 ml. The ultra-sound bath was a product from Bransonic Co. Ltd., Eagle Rd., Danbury, CT 06813, USA. HPLC pump was obtained from Water Ltd. Co. product of type 510, the fraction collector was produced from UltroracLtd. Co. in Sweden (Model: UltracType-7000).