| Reagent | Volume / Reaction (μl) | Volume for 48 Reactions + 10% overage (μl) | Volume for 96 Reactions + 10% overage (μl) |
| 2X TaqMan PreAmp Master Mix (Applied BioSystems) | 2.5 | 132 | 264 |
| 500 nM (10X) pooled primer mixture | 1 | 52.8 | 105.6 |
| Water | 0.25 | 13.2 | 26.4 |
| cDNA | 1.25 | ||
| Total Volume | 5 |
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