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. 2014 Aug 26;(90):51642. doi: 10.3791/51642
SAMPLE MIX Component Volume per Inlet (μl) Volume per Inlet with Overage (μl) Volume for 48.48 Dynamic Array IFC (μl) (120 samples) Volume for 96.96 Dynamic Array IFC (μl) (120 samples)
2X SsoFast EvaGreen Supermix with Low ROX (Bio-Rad, PN 172- 5211) 2.5 3 180 360
20X DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100- 3738) green cap 0.25 0.3 18 36
STA and ExoI treated sample 2.25 2.7
Total 5 6