Solutions	HEPES-buffered sucrose	Protease inhibitors	*Phophatase inhibitors
1 M HEPES pH 7.4	0.32 M sucrose in 4 mM HEPES (pH 7.4)	0.25 mM PMSF (stock 250 mM in ethanol, 1,000x)	10 mM Sodium Fluoride (stock 500 mM, 50x)
4 mM HEPES pH 7.4	0.8 M sucrose in 4 mM HEPES (pH 7.4)	1.5 μg/ml Aprotinin (stock 1.5 mg/ml, 1,000x)	2.5 mM Sodium Pyrophosphate (stock 250 mM, 100x)
ddH2O	1.0 M sucrose in 4 mM HEPES (pH 7.4)	10 μg/ml Leupeptin (stock 10 mg/ml, 1,000x)	1.0 mM b-glycerophosphate (stock 200 mM, 200x)
50 mM HEPES (pH 7.4) 2 mM EDTA	1.2 M sucrose in 4 mM HEPES (pH 7.4)	0.1 mg/ml Benzamidine (stock 100 mg/ml, 1,000x)	5.0 mM Sodium Orthovanadate (stock 500 mM, 100x)
		10 μg/ml Pepstatin (stock 5 mg/ml in ethanol, 500x)