E (kPa)	40% Acrylamide (µl)	BIS (concentration)	2% BIS (µl)	100 mM HEPES (µl)
0.1	625	0.00009	22.5	4225
0.25	625	0.0001	25	4222.5
0.5	625	0.00011	27.5	4220
0.75	625	0.0002	50	4197.5
1	625	0.0003	75	4172.5
1.5	625	0.0004	100	4147.5
2	625	0.0005	125	4122.5
2.5	625	0.0006	150	4097.5
3	625	0.0008	200	4047.5
3.5	625	0.0009	225	4022.5
4	625	0.001	250	3997.5
4.5	625	0.0012	300	3947.5
5	625	0.0014	350	3897.5
5.5	625	0.0016	400	3847.5
6	625	0.0018	450	3797.5
6.5	625	0.002	500	3747.5
7	625	0.0022	550	3697.5
7.5	625	0.0024	600	3647.5
8	1000	0.001	250	3622.5
10	1000	0.0013	325	3547.5
15	1000	0.002	500	3372.5
20	1000	0.0027	675	3197.5
30	1000	0.0037	925	2947.5
40	1000	0.0048	1200	2672.5