Component	Details	Stock	Instructions
10 μM RA	All-trans retinoic acid	5 mM	Resuspend 50 mg RA in 33.3 ml 95% EtOH.  RA is sensitive to heat, light, and air.  Keep in a dark bottle and store at 4 °C for up to 6 weeks.  Use at 1:500 dilution and dilute into differentiation media immediately prior to use
(300.44 g/mol)
1x B-27	B-27 Supplement	50x	Thaw 1-10 ml bottle and aliquot remainder into single-use 1 ml aliquots and store at -80 °C.  Store 10 ml bottles at -20 °C
20 mM KCl	Potassium Chloride	1 M	Add 250 ml water to 18.6 g KCl and sterile filter.  Store at room temperature
(74.55 g/mol)
2 mM db-cAMP	dibutyryl cyclic AMP	1 M	Resuspend full bottle by adding 2.04 ml water to 1 g db-cAMP.  Sensitive to light and moisture.  Store in aliquots of 100 μl or 200 μl at either -20 °C or -80 °C
(491.37 g/mol)
50 ng/ml BDNF	Brain-derived neurotrophic factor (BDNF)	-	Centrifuge vial to get powder to bottom.  Resuspend 10 μg vial in 1 ml Neurobasal + 1x B27 or 5 μg vial in 0.5 ml Neurobasal + 1x B27 to get 10 μg/ml.  Use at 1:200 dilution.  Store working aliquots at -80 °C (ex: 250 μl)
hiFBS	Heat-inactivated Fetal Bovine Serum	-	Aliquot thawed FBS into 50 ml conical tubes.  Heat at 56 °C in a water bath for 30 min.  Remove and freeze working aliquots at -20 °C