Basic Growth Media
Component	Volume for 500 ml	Dilution
EMEM	415 ml EMEM
15% hiFBS	75 ml hiFBS
1x Pen/Strep	5 ml Pen/Strep	1:100
2 mM Glutamine	5 ml Glutamine	1:100
*Keep for 6 weeks maximum
Differentiation Media #1
Component	Volume for 50 ml	Dilution
EMEM	48 ml EMEM
2.5% hiFBS	1.3 ml hiFBS
1x Pen/Strep	500 μl Pen/Strep	1:100
2 mM Glutamine	500 μl Glutamine	1:100
10 μM RA	100 μl RA (5mM stock)	1:500
*Keep for 2 weeks maximum and add RA immediately prior to use
*Do not keep extra media once RA is added - RA is unstable
Differentiation Media #2
Component	Volume for 50  ml	Dilution
EMEM	49  ml  EMEM
1% hiFBS	500 μl  hiFBS
1x Pen/Strep	500 μl Pen/Strep	1:100
2 mM Glutamine	500 μl  Glutamine	1:100
10 μM RA	100 μl  RA (5mM stock)	1:500
*Keep for 2 weeks maximum and add RA immediately prior to use
*Do not keep extra media once RA is added - RA is unstable
Differentiation Media #3
Component	Volume for 50 ml	Dilution
Neurobasal	47 ml Neurobasal
1x B-27	1 ml B-27 (50X stock)	1:50
20 mM KCl	1 ml KCl (1M stock)	1:50
1x Pen/Strep	500 μl  Pen/Strep	1:100
2 mM GlutamaxI	500 μl  GlutamaxI (100x stock)	1:100
50 ng/ml BDNF	250 μl  BDNF stock (10 μg/ml)	1:200
2 mM dibutyryl cyclic AMP (db-cAMP)	100 μl  db-cAMP (1M stock)	1:500
10 μM RA	100 μl  RA (5 mM stock)	1:500
*Keep for 2 weeks maximum and add RA immediately prior to use
*Do not keep extra media once RA is added - RA is unstable