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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Thromb Haemost. 2016 Mar 16;14(4):815–827. doi: 10.1111/jth.13270

Figure 2. Adult and neonatal whole blood platelet aggregation.

Figure 2

Representative CD31-FITC and CD31-eFluor 450 dot plots of adult (A) and neonatal (B) blood at t = 0 min (left) and t = 1 min (right) after treatment with thrombin receptor activator peptide-6 (TRAP-6; 10 μM). Double-labeled events (platelet aggregates) are shown in the upper right quadrant. Representative graph of percent double-labeled events of adult (C) and neonatal (D) platelets stimulated with TRAP-6 (10 μM) or vehicle at 0, 0.5, 1, 2, and 5 min. (E) Fold change of percent double-labeled events normalized to vehicle treatment of adult and neonatal blood stimulated with TRAP-6 (10 μM), collagen related peptide (CRP; 10 μg/mL), epinephrine (10 μM), adenosine 5′-diphosphate (ADP; 10 μM), U46619 (10 μM), ADP+U46619 (10 μM), rhodocytin (300 nM), or calcium ionophore A23187 (10 μM). Data are represented as mean ± SEM; Nadult = 3 and Nneonate = 4; *P < 0.05 with respect to vehicle treated samples; #P < 0.05 with respect to adult samples.