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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Thromb Haemost. 2016 Mar 16;14(4):815–827. doi: 10.1111/jth.13270

Figure 3. Adult and neonatal platelet adhesion and aggregation under static conditions.

Figure 3

Representative differential interference contrast images and corresponding fluorescent CD41a-PE and CD62P-FITC images of adult and neonatal platelet aggregates formed on coverslips coated with 100 μg/mL von Willebrand factor (VWF) (A) or 100 μg/mL fibrillar collagen (B). Adult and neonatal citrated whole blood were pretreated with vehicle, thrombin receptor activator peptide-6 (TRAP-6; 10 μM), collagen related peptide (CRP; 10 μg/mL), adenosine 5′-diphosphate (ADP; 10 μM), U46619 (10 μM), ADP+U46619 (10 μM), epinephrine (10 μM), ristocetin (1 mg/mL), calcium ionophore A23187 (10 μM), or rhodocytin (300 nM) for 10 min prior to coverslip incubation. Mean volume of adult and neonatal platelet aggregates positive for CD62P-FITC formed on coverslips coated with VWF (C) or fibrillar collagen (D). Data are represented as mean ± SEM; Nadult = 3 and Nneonate = 3; *P < 0.05 with respect to vehicle treated samples.