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. Author manuscript; available in PMC: 2017 Apr 28.
Published in final edited form as: J Control Release. 2016 Feb 26;228:38–47. doi: 10.1016/j.jconrel.2016.02.036

Figure 1. Construction and validation of megakaryocyte-specific lentiviral expression vectors.

Figure 1

(a) Schematic of lentiviral vectors for expression of EGFP and TRAIL in megakaryocytes. (b) Flow cytometry detection of transgene expression after transduction of Ubc-EGFP, αIIβ-EGFP, Ubc-TRAIL and αIIβ-TRAIL in promegakaryocytic cell line Dami and human embryonic kidney cell line 293T, respectively. (c) Immunofluorescence staining of surface-bound TRAIL in Ubc-TRAIL-and αIIβ-TRAIL-transduced Dami and 293T cells. Nuclei were stained with DAPI (blue).