Figure 4. Multilayered scaffolds based on PLT32o grids maintain their 3D structure and retain exogenous heart cells in vivo.
(A-C) Scaffolds based on SSoff grids of PLT32o implanted without exogenous heart cells; (D-I) Scaffolds based on SSoff grids of (D-F) PLT32o or (G-I) PGS implanted (D-I) with exogenous heart cells. Explants were harvested after (A,C,D,F,G,I) 31 days or (B,E,H) 7 days. Stains: (A,D,G) H&E; (B,C,E,F,H,I) cardiac troponin T (cTnT). Cytoplasmic cTnT appears dark brown; nuclei appear blue; polymer appears white. Scale bars: 500 μm (A, D, G); 200 μm (B, C, E, F, H, I); 30 μm (B1, C1, E1, F1, H1, I1).
