Fig. 3.

Reduced CNS accumulation of virus-specific IgG⁺ ASC in CXCL13^−/− mice. Numbers of total and virus-specific IgM (A and B) or IgG-secreting (C and D) ASC in brain (A and C) or spinal cord (B and D) measured by ELISPOT assay (left column). Data are representative of 2–3 independent experiments with similar trends in each experiment. To better reflect relative differences between WT and CXCL13^−/− frequencies, data in the right column show the mean percentage changes in frequencies ±SEM calculated relative to levels in WT mice, which were set to a baseline value of 100. Data represent 2–3 independent experiments, each comprising pooled brain or spinal cords of 3–4 mice. Significant differences between WT and CXCL13^−/− mice determined by the unpaired t test are denoted by ^**(p < 0.01) and ^***(p < 0.005).