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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Mol Nutr Food Res. 2016 Feb 18;60(4):858–870. doi: 10.1002/mnfr.201500814

Figure 1. Time- and dose-response effects of EGCG on cell cytotoxicity and nuclear accumulation and mRNA expression of Nrf2 in HC-04 human hepatocytes.

Figure 1

HC-04 were treated with 0-5 μM EGCG for up to 2 h as described under Methods. A) Representative Western blot of Nrf2 from isolated nuclear fractions of HC-04 cells following 2 h incubation with EGCG or 100 μM t-butylhydroperoxide (positive control). B) HC-04 cells were treated for up to 2 h with 0.1-5 μM EGCG before isolating total RNA using Trizol and reverse transcribing for RT-PCR analysis of Nrf2 mRNA expression. C) LDH leakage and D) cell viability were assessed using spectrophotometric kits as described under Methods following treatment of HC-04 for 2 h with 0-5 μM EGCG. Shown are mean responses of experiments performed in triplicate. Data were analyzed by 1- or 2-way ANOVA as appropriate. There were no statistically significant time- or concentration-dependent effects of EGCG. Abbreviations: EGCG, epigallocatechin gallate; LDH, lactate dehydrogenase; Nrf2, nuclear factor erythroid-2-related factor-2.