RNA was isolated using Trizol and reverse transcribed for RT-PCR analysis for TNFR1 and TLR4 using the primers described in Table 1. Data (means ± SEM, n = 10-12 mice per group) were analyzed by 2-way ANOVA with Newman-Keuls post-test to evaluate main and interactive effects. Groups without a common letter are significantly different (P<0.05). Abbreviations: GTE, green tea extract; Nrf2, nuclear factor erythroid-2-related factor-2; TLR4, toll-like receptor 4; TNFR1, tumor necrosis factor receptor-1; WT, wild-type.