Morphology in vitro and cell growth rate of LSCs in the different culture systems. Morphology of the control (A, B), 2D-SC (C), 2D-CC (D), 3D-CC (E, F), fibrin 3D-CC (G, H) and cell population doubling (I). The dashed circle in H indicates clusters of cells with a LSC-like morphology. Scale bar indicates a distance of 100 μm.
Abbreviations: 2D-SC, single LSCs in the direct system with BMSCs; 2D-CC, LSC clusters in the direct system with BMSCs; 3D-CC, LSC clusters in the 3D system with BMSCs; fibrin 3D-CC, LSC clusters in the fibrin 3D system with BMSCs.