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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Eur J Immunol. 2016 Jan 20;46(4):897–911. doi: 10.1002/eji.201546015

Figure 3. Kinetics of cell recruitment to the site of infection.

Figure 3

C57BL/6 mice were infected in the ear dermis with 1000 LmFn or LmSd metacyclic promastigotes and ear tissues were processed at different times post-infection to follow the kinetics of cell recruitment. (A) Representative dot plots of ear derived dermal cells. Subpopulations of myeloid (CD11b+) cells are defined by the following markers: Ly6Cint Ly6G+ (neutrophils); Ly6C−Ly6G− (primarily resident dermal macrophages and DCs); Ly6ChiLy6G− and Ly6CintLy6G− (variable proportions of inflammatory monocytes and monocyte derived macrophages and DCs). T cells were identified by their expression of TCRβ and further separated in CD4+ and CD8+ T cells. (B) The number of myeloid cells subsets are represented over the course of infection. (C) The number of CD4+ and CD8+ T cells are represented over the course of infection. Values shown are the means ± SD of 8 samples (4 mice, 8 ears) at each time point.