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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Eur J Immunol. 2016 Jan 20;46(4):897–911. doi: 10.1002/eji.201546015

Figure 4. IL-1 expression in ear dermal cells.

Figure 4

(A) C57BL/6 mice were infected in the ear dermis with 1000 LmFn or LmSd metacyclic promastigotes and total mRNA was isolated from the ear tissue at the indicated times during infection, reverse transcribed and analyzed by real-time PCR for expression of IL-1α and IL-1β. Target genes were normalized to endogenous control, and the values shown are the fold increase relative to expression in naïve ears. Data are represented as means ± SD of 8 samples (4 mice, 8 ears) at each time point. The results are representative of 2 independent experiments. (B) Ear tissues were processed for IL-1α and IL-1β intracellular staining at wk 4 post-infection. Representative dot plots are shown. The numbers on the plots represent the percentage of IL-1α and IL-1β positive cells in the ears as a percentage of the total number of viable cells recovered. (C) The number IL-1α+ and IL-1β+ cells 4 weeks post infection are represented as means ± SD of 10 ears, 5 mice per group. * p<0.05. (D) IL-1β intracellular staining at wk 4 post-infection. Shown are the dot plots gated on total CD11b+ cells (top), or CD11b+IL-1β+ cells (bottom) and stained for Ly6G and Ly6C. The graph shows the frequency of each subset staining positive for IL-1β as a percentage of the total IL-1β positive cells. Means ± SD, 10 ears, 5 mice.