Figure 5. IL-1β and Nlrp3 inflammasome activation are required for non-healing infection and severe pathology due to LmSd.
Lesion development and pathology score over the course of infection with 1000 LmSd metacyclic promastigotes in the ear dermis in C57BL/6 (WT) and (A) IL-17A−/−, (B) CXCL1−/−, (C) IL-1R−/−, with parasite burden in the ear lesion over the course of infection also shown, (D) IL-1R−/−, IL-1β−/−, Caspase1/11−/− and ASC−/− mice, with (E) total number of CD11b+ and CD11b+Ly6Cint Ly6G+ (PMN) cells recovered from the ear dermis 4 wks post-infection, and (F) Nlrp3−/− mice, with parasite burdens in the ear lesion and dLN at 16 wks. Data are means ± SD of 4–10 ears, 4–5 mice per group. * p<0.05 comparing lesion size, pathology score, parasite load, or inflammatory cell numbers in WT and KO mice. The results are in each case representative of 2 independent experiments.