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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Eur J Immunol. 2016 Jan 20;46(4):897–911. doi: 10.1002/eji.201546015

Figure 6. Cytokine responses by dLN cells associated with resistance to LmSd infection in IL-1R−/− and Nlrp3−/− mice.

Figure 6

C57BL/6 and IL-1R−/− mice were infected in the ear dermis with 1000 LmSd metacyclic promastigotes. Nine wks post-infection, dLN cells were harvested, stimulated for cytokine production and analyzed by intracellular staining and ELISA. (A) Representative dot plots showing the frequency of dLN CD4+ T cells staining positive for selected cytokines following a 4 hr stimulation with PMA/ionomycin. Bar graphs show the frequency of CD4+ T cells staining positive for IFNγ, IL-4, IL-10, or doubly positive for IFNγ and IL-10. IL-17 staining cells were not detected. Data are means ± SD of 3–7 mice per group. (B) Concentration of released cytokine in culture supernatants following antigen stimulation of dLN cells recovered from wt and IL-1R−/− mice at 9wks, or (C) Nlrp3−/− mice at 16 wks. Data are means ± SD of 4–8 mice per group; * p<0.05.