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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Eur J Immunol. 2016 Jan 20;46(4):897–911. doi: 10.1002/eji.201546015

Figure 8. Neutropenic Genista mice are resistant to infection with LmSd.

Figure 8

(A) Lesion development in C57Bl/6 and Genista mice over the course of infection in the ear dermis with 1000 LmSd metacyclic promastigotes. Data are means ± SD 4–5 mice per group; * p<0.05. One of two representative experiments is shown. (B) Parasite burden in the ear lesion and dLN at 11 wks. Results shown are the mean ± SD of 3–4 mice/group; * p<0.05. (C) Bar graphs show the total number of CD45+ cells and the frequency of myeloid cell subsets and CD4+ T cells recovered from the ear dermis at 5 wks. Data are means ± SD of 3–4 mice per group; * p<0.05. One of two representative experiments is shown. (D) IL-1β intracellular staining at wk 5, with representative dot plots showing the percentage of IL- IL-1β positive cells in the ears as a percentage of CD11b+ cells. Bar graphs show the frequency and number of IL-1β+ cells at 5 wks, represented as means ± SD, 3 mice per group. One of two representative experiments is shown. (E) The frequency of CD4+ T cells recovered from dLN or ear at 5 wks post-infection staining positive for IFNγ following a 4 hr stimulation with PMA/ionomycin. Data are means ± SD of 4 mice per group; * p<0.05.