. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: J Neurochem. 2016 Mar 15;137(2):287–298. doi: 10.1111/jnc.13557

Table 1.

Binding affinity of gp120s to TUBB3, tubulin dimer and assembled MTs.

Ligand Analyte	TUBB3		Tubulin dimer		Assembled MTs
Ligand Analyte	U-value	K_D (nM)	U-value	K_D (nM)	U-value	K_D (nM)
gp120ADA	3	10.3	4	11.2	5	12.0
gp120ADA	2	10.3	2	11.2	3	12.0
gp120IIIB	2	7.5	2	12.3	7	10.0
gp120IIIB	2	7.5	4	12.3	4	10.0
gp120MN	12	740	26	79.2	7	62.7
gp120MN	15	740	5	79.2	7	62.7
Tat	no specific binding

Recombinant TUBB3, bovine tubulin dimer, and assembled MTs were immobilized on a Biacore® CM5 chip by amine coupling. Different concentrations of gp120s (from 3.13 to 100 nM) or Tat (from 1.6 nM to 100 nM) were injected over the surface in triplicates in each experiment. The kinetics of interaction was analyzed using Biacore T200 instrument as described in Materials and Methods. K_D was calculated from two independent experiments, in triplicates.