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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Neurochem. 2016 Mar 15;137(2):287–298. doi: 10.1111/jnc.13557

Table 2.

Sequence and characteristics of tubulin isoforms used for dot blot analysis.

Tubulin
isoform		 Sequence Expression Distinctive
feature		 Binding
to gp120
α1/β1 441EGEGEEEGEEY451 widely expressed tyrosinated isoform No
α1/β1 441EGEGEEEGEE450 widely expressed detyrosinated isoform No
α1/β1 441EGEGEEEGE449 expressed exclusively in neurons Δ2 isoform1 Yes
α4 441EDEDEGEEY449 widely expressed tyrosinated isoform No
α4 441EDEDEGEE448 widely expressed detyrosinated isoform No
α4 441EDEDEGE447 widely expressed Δ2 isoform1 No
β2A/2B 437EEEEGEDEA455 widely expressed; highly abundant in neurons No
β3 438EDDEEESEA446 expressed exclusively in neurons Yes
1

irreversible derivative of detyrozination

Binding of gp120 (IIIB or ADA) to the indicated sequences of CTT tubulin isoforms was performed by dot blot as described in Materials and Methods.