Figure 7.

Microscopic observation of H&E-stained paraffin section of kidneys and livers of immunized mice against CA (2×10⁶) infection. One week after the third time immunization, all mice were challenged with CA. Histological sections (3–5 mm) of kidneys and livers of each mice were stained with H&E, observed with microscope and then photographed by digital cameras. (a) Livers from mice immunized with rpSap2. Magnification: 400×, (b) Livers from mice immunized with EPSP. Magnification: 400×, (c) Livers from mice immunized with WTP. Magnification: 200×, (d) Livers from mice immunized with PBS. Magnification: 200×, (e) Kidneys from mice immunized with rpSap2. Magnification: 400×, (f) Kidneys from mice immunized with EPSP, Magnification: 400×, (g) Kidneys from mice immunized with WTP, Magnification: 200×, (h) and (i) Kidneys from mice immunized with PBS, Magnification: 200×. These images suggested that immunization with EPSP (b, f) or rSap2 (a, e) protected the mice against CA infection in visceral injury compared to PBS group (d, h, i) and WTP group (c, g). The arrows in (c), (d), (g) and (h) indicate infiltration of inflammatory cells, and those in (i) indicate protein tubes.