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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Ann Rheum Dis. 2015 Aug 7;75(6):1145–1151. doi: 10.1136/annrheumdis-2015-207509

Figure 3. PUF60 expression in skin and salivary gland.

Figure 3

A & B: Immunoblots performed on lysates made from frozen tissues. Equal protein amounts of lysates made from skin biopsies (dermatomyositis and control subjects, panel A lanes 1–12) and salivary glands (SS and control subjects, panel B lanes 13–23) were immunoblotted with a polyclonal anti-PUF60 antibody. Vinculin (skin) and -actin (salivary gland) were included as loading controls. C & D: Immunohistochemical PUF60 staining. Skin (Panel C, control and dermatomyositis) and salivary gland (Panel D, control and SS) paraffin sections were stained with a rabbit anti-PUF60 antibody. Representative images are shown. In Panels C & D, the scale bar represents 50 μM in the 2 upper images, and 20 μM in the lowest images. No staining was detected when controls were performed by incubating serial sections with rabbit IgG instead of the rabbit anti-PUF60 polyclonal antibody (data not shown).